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Caution : Avoid exposure to phenol vapors during use. 40 Aravind’s Atlas of Fungal Corneal Ulcers TECHNIQUES OF MICROSCOPIC EXAMINATION Principle The simplest technique consists in placing a fragment of the colony between a slide and cover slip. This approach is rapid and often adequate for identification. Delicate fungal structures, however, are often broken by this process, rendering identification difficult. The adhesive tape technique assists in maintaining the integrity of fungal structures by fixing them on the adhesive surface of a piece of transparent (Not frosted) tape.

Macroscopic and Microscopic Characteristics of Ocular Fungal Isolates Fig. 2: Acremonium species. Oblong conidia accumulating at the apices of narrow Phialides 45 46 Aravind’s Atlas of Fungal Corneal Ulcers ALTERNARIA Pathogenicity Occasional agents of keratitis, other infections include onychomycosis, ulcerated cutaneous infection, chronic sinusitis and rare cases of deep infection have also been reported in the immunocompromised patient. Ecology Alternaria is a cosmopolitan dematiaceous (phaeoid) fungus commonly isolated from plants, soil, food, and indoor air environment.

C. Quality control: Appearance: Deep blue liquid, slightly viscous Usage : A mounting medium for microscopic examination. Caution : Avoid exposure to phenol vapors during use. 40 Aravind’s Atlas of Fungal Corneal Ulcers TECHNIQUES OF MICROSCOPIC EXAMINATION Principle The simplest technique consists in placing a fragment of the colony between a slide and cover slip. This approach is rapid and often adequate for identification. Delicate fungal structures, however, are often broken by this process, rendering identification difficult.

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