By Dr. med., Prof. Herbert Begemann, Dr. med., Priv.-Doz. Johann Rastetter (auth.)
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Promyelocyte. 3. Myelocyte. 4. Metamyelocyte. 5. Stab cell. 6. Segmented neutrophil (polymorph). 7. Monocyte with fine peroxidase positive granules. 8. Lymphocyte (peroxidase negative). 48 Rarely degenerated leucocytes due to irritation are found in the peripheral blood (cell 17-20). They usually have the same size as the segmented types but are frequently considerably smaller (4-8 /lm). Their cytoplasm is rather more basophil than that of polymorphonuclears, granulation coarser and often indistinct.
Serosa cells, particularly frequently found on splenic puncture. They derive from the single endothelial layer covering the spleen. The finely reticulated chromatin of the nucleus is typical. Nucleoli are demonstrable in only a few cases. The cytoplasm is basophil and contains fine reddish violet deposits. Granules are not seen. These cells often show multiple nuclei. 30 4 '. , ;;. , I .............. , , . ,' ... ,:. -' Fig. 5. Plasma cells ""' 1250 x They typically have a basophil cytoplasm and an eccentric nucleus.
The nucleus reveals a dense reticular chromatin structure. As a rule several, up to 5, pale blue nucleoli are present which however are poorly demarcated and disappear on maturation of the cell. Proerythroblasts, like all erythroid cells, tend to be multinucleate, but less so than plasma cells. The bright area in the cytoplasm surrounding the nucleus is characteristic (cell 3, 8, 10, 11, 13); this appears as a fmely granular zone on phase contrast microscopy. Deposition of haemoglobin commences near the nucleus where it is at first seen as a light perinuclear zone (cell 1, 2, 5), eventually spreading throughout the cell, thus forming the transition to polychromatic types (cell 17-20).