Download Clinical Impact of Bone and Connective Tissue Markers by E. Lindh (Eds.) PDF

By E. Lindh (Eds.)

For a few years, biochemical checks were well-known for the analysis, category, and tracking of bone and connective tissue illness. although, shortcomings when it comes to the specificity and sensitivity of such assessments have triggered continuous wondering approximately their medical usefulness. This ebook, in line with a gathering equipped together by way of the college of Uppsala and Pharmacia Diagnostics AB, experiences our Read more...

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Wieslander, J. and Paulsson, M. (1985). Biochem. J. 225, 95-106. , Paulsson, M. and Hedbom, E. (1986). / . biol. Chem. 261, 13866-13872. , Sandfalk, R. and Vogel, K. (1985). Biochem. J. 230, 181-194. Maroudas, A. (1979). In Adult Articular Cartilage (Ed. R. Freeman), pp. 215-290. Pitman Medical. , Heinegârd, D. and Engel, J. (1988), Biochem. , 253, 175-185. Paulsson, M. and Heinegârd, D. (1981). Biochem. J. 197, 367-375. Paulsson, M. and Heinegârd, D. (1982). Biochem. J. 207, 207-213. , Olsen, B.

230, 181-194. Maroudas, A. (1979). In Adult Articular Cartilage (Ed. R. Freeman), pp. 215-290. Pitman Medical. , Heinegârd, D. and Engel, J. (1988), Biochem. , 253, 175-185. Paulsson, M. and Heinegârd, D. (1981). Biochem. J. 197, 367-375. Paulsson, M. and Heinegârd, D. (1982). Biochem. J. 207, 207-213. , Olsen, B. R. (1986). Biochem. J. 237, 923-925. Scott, J. and Orford, C. (1981). Biochem. J. 197, 213-216. I. and Mayne R. (1988). / . Cell Biol. 106, 991-997. , Paulsson, M. and Heinegârd, D. (1984).

Following 10 min at a flow rate of 50 ml/min, the perfusion medium is changed to a buffer containing collagenase. A t this point the liver is dissected out, and perfusion is continued for an additional 10 min with recirculation of the enzyme solution. After the collagenase-perfusion, the liver cells are dispersed and can be recovered as a single-cell suspension, which is centrifuged at low speed to sediment most of the P C . T h e supernatant, containing the bulk of the n o n - P C (that is, mostly sinusoidal cells) is concentrated by centrifugation, and layered on top of a two-step Percoll gradient (p = 1-040 and 1-065).

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