Download Computer applications in biotechnology : a postprint volume by A Munack; K Schügerl; International Federation of Automatic PDF

By A Munack; K Schügerl; International Federation of Automatic Control

The sixth laptop functions in Biotechnology (CAB6) convention was once a continuation of two sequence of occasions: the IFAC symposia on Modelling and regulate of Biotechnical approaches and the foreign meetings on computing device purposes in Fermentation know-how. This convention supplied the chance for either side, major researchers and business practitioners, during this interdisciplinary box to replace new principles and expertise; recommendations and strategies. This postprint quantity comprises all these papers which have been provided on the convention.

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Prior to initia­ tion of this control strategy, the cultivation is sub­ jected to a batch phase, in which both substrate and oxygen are available in excess, fig. 1 . After some time the dissolved oxygen concentration decreases to a set point of 10 % p02, after which further decrease is hindered through control of stirrer speed. Following the exhaustion of available pri­ mary substrate, the metabolic activity decreases . Consequently , the dissolved oxygen concentration rises, because the controller is only active, if p02 is not higher than the set point.

2. 4 mL). Table 1 Factorial experimental design THE CASE STUDY APPLICATION The chromatographic process min max centre �I �2 system (Beckman Instruments Inc, USA) comprising an autosampler, dual pump and ultraviolet (UV) detector Jinked to a central IBM-PC. The synthetic mixture separated by the column comprised of myoglobin, ovalbumin and BSA (Sigma Chemical Co, USA) which represented the monomer, dimer, and trimer respectively of the industrial scale system. The ratio of these proteins was chosen as 1 7 :2: 1 and made up in the mobile phase.

High flow rates give a positive deviation in the peak at 60 mins. 56% of the total variation in X. The validation procedure was extended to the 9 independent cluster models and it was found that either a 2 or 3 component model was sufficient. Since a priori information was available by using an experimental design approach, the removal of outliers was not necessary prior to validation of the disjoint cluster models. 3 - - - - I ! ' - I I j - - - - - I - - - II I - - - - I I ----- I I • I I I I - - - - , - - - - r - - - - , - - - - r - -• - � - - -· � - - - �� - - - - � - - - - � - - - • _ _ _ _ - - - - • • ,...

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